Mitochondrial respiration measurement modified for Drosophila.

Authors: James Jiayuan Tong and Douglas C. Wallace
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Pierce spin chromatography purification protocol with protein G and protein A

Authors: Molecular Methods Database
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The Dual tag microarray readout is used for high precision readout of reporter nucleic acids comprising two tag motifs, in the 3’ and 5’end respectively.

Authors: Own Document
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Proximity ligation assay is a protein-analyzing method for detection and characterization of proteins with high specificity and sensitivity.

Authors: Own Document
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This method is used for detecting and genotyping mitochondrial DNA (mtDNA) in fixed cell materials. The method was first described in 2004 (Larsson et al, 2004) and utilizes padlock probes amplified by rolling circle amplification (RCA) to detect and visualize localized mtDNA molecules in situ.

Authors: Own Document
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This method encodes nucleic acid targets into single stranded reporter molecules comprising tag motifs in the 3’ and 5’ end. These reporter molecules can then be decoded with very high precision and sensitivity by the dual tag microarray readout platform. The method requires design of a padlock probeset comprising two separate tag motifs in each probe along with the appropriate restriction enz...

Authors: Own Document
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T vectors are linear vectors with a 3´ terminal thymidine at each end. These single 3´-T overhangs allow efficient ligation of a PCR product into the plasmids by preventing recircularization of the vector and providing a compatible overhang for PCR products with a single deoxyadenosine at the 3´-ends. With an additional A tailing step, a PCR product generated by a proofreading DNA polymerase ca...

Authors: Own Document
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This protocol describes the sensitive and specific detection of Aujeszky’s disease virus (ADV), African swine fever virus (ASFV), porcine circovirus type 2 (PCV2) and porcine parvovirus (PPV). The assays target the gD gene (ADV), the 9GL gene (ASFV), the Rep gene (PCV2) and the VP2 gene (PPV), amplifying PCR products of 110, 264, 263 and 130bp respectively. Each assay has a detection limit of 2...

Authors: Unknown User
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This RT-LAMP assay provides a number of benefits for the diagnosis of SVD, since the assay is sensitive and rapid, and the isothermal amplification strategy used is not reliant upon expensive equipment it is particularly suited for “front line” diagnosis of SVD in modestly equipped laboratories, in field stations or in mobile diagnostic units.

Authors:  Mikhayil Hakhverdyan
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This RT-LAMP assay provides a number of benefits for the diagnosis of SVD. Since the assay is sensitive and rapid, and the isothermal amplification strategy used is not reliant upon expensive equipment it is particularly suited for “front line” diagnosis of SVD in modestly equipped laboratories, in field stations or in mobile diagnostic units.

Authors:  Mikhayil Hakhverdyan
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