Protocol for preparation of plasma

Summary

Plasma is the liquid transparent upper fraction obtained after centrifugation of whole blood collected in tubes with different anti-coagulants; EDTA, Li-Heparin or citrate. Plasma contains a major part of all human proteins and metabolites. Citrate plasma is primarily used for analysis of coagulation factors. Different citrate tubes are used depending on the analysis.

• Blood is drawn from the vein (venipuncture) into standardised Vacutainer Venous Blood Collection tube containing different anticoagulants; EDTA, Li-Heparin with or without gel plug or in a citrate tube.

• The tube must be mix gently by slowly inversion (around 10 times)

• Centrifuge EDTA and Li-Heparine tubes at 2000g for 10 min.

• Centrifuge citrate tubes at 2500g for 20-30 minutes in order to minimise the content of thrombocytes. To ensure thrombocyte-free citrate plasma, a double centrifugation step is recommended.

• The supernatant (plasma) is aliquotted into a barcoded polypropylene tube for long term storage.

• A general recommendation is cold handling, 4-8ºC, of plasma.

• Plasma should be stored at – 80 ºC or lower for long term storage.

Citation

Ortega-Paino, Eva. Protocol for preparation of plasma. The Molecular Methods Database. 2013/12/20

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Comments

Cold handling?
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Yes, it is better.
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Where?
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Test
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Bottom
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