Preparation of serum from whole blood

Summary

Serum is the liquid fraction obtained from whole blood after coagulation. This fraction contains all proteins and metabolites present in plasma with the exception of coagulation factors. This is the recommended protocol from the Biobank at Karolinska Institutet and BBMRI.se

• Blood is drawn from the vein into a Vacutainer Venous Blood Collection tube with or without clot activating/gel tubes• Leave the blood rest at room temperature in an upright position for 30 min for coagulation.• Centrifuge the tube at 2000g for 10 min preferably at 4-8ºC
• The supernatant (serum) is aliquoted into barcoded polypropylene storage tubes.
• Serum should be stored at -80°C for long-term storage Caution: Avoid delayed transport time, e.g > 4 hrs in room temperature. A general recommendation, if samples are to be transported before aliquotation, is to use a gelplug and centrifuge the tube before transport.

Citation

Ortega-Paino, Eva. Preparation of serum from whole blood . The Molecular Methods Database. 2013/12/20

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