Padlock probes

Molecular padlock probes are oligonucleotides designed to circlularize by hybridization and ligation to a DNA target. The circles formed after a good target match can be amplified by rolling circle amplification (RCA), once or by several cycles - a process referred to as circle-to-circle amplification (C2CA). The products can be visualized by fluorescent labeling and microscopy - in situ or in a flow cell - or detected by other readouts, such as microarrays, biosensors or colorimetric reactions. Some features of padlock probes include selectivity for single nucleotide polymorphisms (SNPS) and high degree of multiplexibility. Probes circularize and amplify virtually independently of each other.

Can be applied for various diagnostic purposes, such as tests for infectious disease or cancer tissue analysis.

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