DNA extraction and storage

Biological materials collected in harsh environments like during archaeological excavations, at a crime scene, at mass disasters, in museums or non-invasively in the field constitute highly valuable source genetic information. However, poor quality and limited quantity of the DNA extracted from these samples can be extremely challenging during further analyses. This group is dedicated towards collecting and sharing methods related to this field of research.

Latest News

Summary or abstract: 
This protocol describes the workflow for manual DNA extraction at the KI Biobank.
Author(s): KI Biobank
Tuesday, January 26, 2016 - 18:24
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Summary or abstract: 
Lepidium campestre has proven to be one of the more difficult plant species for DNA isolation. Here we describe a method that has proven to produce good and clean DNA for RAD sequencing purposes. There are several stages to the protocol. First the leaf samples are pulverized and proteins are broken down. Second stage is the actual extraction of DNA which should follow directly after the pulverization and protein breakdown. Third stage comprises of quality control and RNA removal. This is essential if the samples are intended for example RAD sequencing.
Author(s): Fernando Lopes Pinto and Tytti Vanhala
Friday, September 4, 2015 - 10:02
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Summary or abstract: 
A practical guide for collecting different types of biological material. Provides a method of collecting, storing and labeling, depending on the type of tissue and the selected method of storage. It is published as a supplementary material to the December-February Special Issue on Biobanking in Emerging countries in the journal Biopreservation & Biobanking.
Author(s): Magdalena Bus and Marie Allen
Sunday, April 27, 2014 - 01:35
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Summary or abstract: 
The protocol constitute a guideline for decontamination of laboratory and basic tools used during the work with the biological material for DNA analysis. It is published as a supplementary material to the December-February Special Issue on Biobanking in Emerging countries in the journal Biopreservation & Biobanking.
Author(s): Magdalena Bus and Marie Allen
Monday, April 21, 2014 - 01:42
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Summary or abstract: 
This protocol is developed and optimized for DNA extraction and storage of samples collected from hair.
Author(s): Magdalena Bus
Friday, April 25, 2014 - 19:27
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Summary or abstract: 
This protocol is developed and optimized for DNA extraction and storage of samples collected from bones.
Author(s): Magdalena Bus and Marie Allen
Saturday, April 19, 2014 - 08:10
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Summary or abstract: 
This protocol is developed for DNA extraction and storage of samples collected from formalin fixed paraffin-embedded (FFPE) tissue samples.
Author(s): Magdalena Bus and Marie Allen
Sunday, April 20, 2014 - 12:51
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Summary or abstract: 
This protocol is developed and optimized for DNA extraction and storage of samples collected from swabs
Author(s): Magdalena Bus
Tuesday, April 22, 2014 - 02:15
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Summary or abstract: 
The protocol constitute a guideline for decontamination of laboratory and basic tools used during the work with the biological material for DNA analysis. It is published as a supplementary material to the December-February Special Issue on Biobanking in Emerging countries in the journal Biopreservation & Biobanking.
Author(s): Magdalena M. Bus and Marie Allen
Monday, March 3, 2014 - 12:51
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