0352 Guidelines working in the lab - KI Biobank

Summary

To describe the general guidelines for laboratory work at KI Biobank, in order to achieve a good and safe working environment.

1. Purpose

To describe the general guidelines for laborative work at KI Biobank, in order to achieve a good and safe working environment.

2. Scope

The instruction includes general guidelines, risks at the lab, waste management, and guidelines how to work with possibly contaminated blood. It also includes work in the lab outside normal working hours, such as work during the weekend.

3. Responsibility

The nearest boss is responsible for updating these guidelines regularly. All personnel in the lab is responsible for following the guidelines. The safety officer is responsible for educating new personnel about contamination risks, transmission routes, and safety measures, including vaccinations.

4. General guidelines

New personnel should get an introduction to the safety prescriptions by the safety officer before starting any work in the lab. They should also be offered a vaccination against Hepatitis B.

4.1.1 Working environment and accidents

  • Go through the escape plan and make sure what to do in case of a fire, and where the fire extinguishers and nearest fire alarm button are placed.
  • Make sure you know where eye-showers and emergency showers are placed.
  • In case of an accident, inform your nearest boss, the lab manager, and the safety officer.
  • The working environment binder includes routines used when reporting a work injury, issues about the work environment, etc. The binder can be found in the room of the safety officer, room 1433.
  • Information can also be found on the intranet: http://internwebben.ki.se/sv/tillbud-och-arbetsskador-pa-laboratoriet
  • One should try to always work at least two persons together in case of an accident.
  • If something is not working as expected, the lab manager should be informed.
  • If locked out and without card, call the lab on your mobile phone or entry phone. If the only one left in the lab, call the guard on the entry phone.

4.1.2 Individual protection

  • Watch your hand hygiene. Use soap, water, and ethanol to wash your hands.
  • It is not allowed to eat or drink in the lab.
  • Use protective coat and gloves. Safety goggles and mouth protection should be used when risk of splashing. Change your gloves often to get a good protection, as the protection of the gloves is decreased with skin contact.
  • Fetch a new pack of gloves when you take the last glove in the pack. Make a note on the white board in room 1433 when only 10 packs remain.
  • Take off your gloves when using the phone.
  • Use thicker gloves when working with the -80°C freezers or with liquid nitrogen.
  • Use safety goggles when working with liquid nitrogen.
  • When working with dangerous compounds, use the fume hoods.
  • Always leave the working benches tidy and clean when finishing every day, and throw your waste in the appropriate container.
  • Use the absorbing bench paper when spillage is possible.
  • Pull down the window of the fume hood as much as possible while working and when you leave it.
  • Never use any apparatus before getting an instruction.

4.1.3 Other

  • When the 70% EtOH finishes, dilute from the 95% EtOH, and fill up.
  • When the waste container is full, seal it, fill in the information, and replace it with a new one.
  • The reception attends the lab phone during working hours (8:00-16:30). If one cannot attend due to meetings, etc., the responsibility should be left to someone else.

4.2 Risks in the lab

In the lab there are a few chemicals considered toxic. Always read the label on the flasks/package to be aware of the potential danger of the substance. It is not only your own health that is at risk, but also that of your colleagues. In most cases the nature of the risk can be found; if corrosive, carcinogenic, irritating, etc.

A binder with information on the dangerous chemicals can be found in room 1433.

Other risks in the lab are while working with freezers and liquid nitrogen. See 4.1.2 and 4.2.1.

4.2.1 Liquid nitrogen

See internal documents at the homepage of KI:

http://intra.ki.se/staff/work_environment2/documents/internal_se.html

4.3 Waste management

There are different types of waste in the lab: chemical waste, glass waste, plastics, cardboard, papers, and sharps waste.

4.3.1 In general

All packages should be empty, cleaned, dry, and as flat as possible when they are thrown away. All waste containers should be properly labelled, and overfilling should be avoided.

4.3.2 Chemical waste

No corrosive, toxic, or flammable compounds should be thrown in the sink. Solvents like isopropanol and ethanol can be poured in the sink if rinsed with water. A maximum of 3 liters 10% solutions can be poured in the sink per day, provided that there´s no risk for ignition or inhaling.

4.3.3 Glass waste

Non-contaminated glass waste is rinsed and placed on a trolley in the washing room, for further transport to glass recycling in the garbage room in the basement.

4.3.4 Plastic waste

Plastic dunks are to be rinsed and placed on a trolley in the washing room, for further transport to the plastic recycling in the garbage room in the basement. If contained blood waste they should be placed in a waste container for contaminated material. Fill it up with empty tubes like EDTA-tubes and Gentra-tubes, before it is thrown away. Styrofoam is placed in the container for combustible waste in the basement.

4.3.5 Cardboard waste

Cardboard boxes are folded and placed on a trolley in the washing room, for further transport to the garbage room in the basement.

4.3.6 Paper waste

Paper waste is thrown in the paper recycling.

4.3.7 Contaminated waste

See instruction 0279 Sharps waste for information.

4.4 Guidelines working with possibly contaminated blood           

There is a risk for contamination when working with blood,or secretions containing blood, infected  by HIV, hepatitis B or C or other blood-borne viruses. Blood products, tissue, and other material that has been in contact with blood/tissue without being purified from infection, also counts as blood. Therefore, plasma, serum, spinal fluid, sputum, primary cell cultures, etc, as well as all material such as pipette tips and needles, count. Treat all material as if contaminated!

Also see:

http://internwebben.ki.se/sites/default/files/blood_rules_ki_rev_enl_afs_2012_7_0.pdf

  • Only personnel trained on the contamination risks, transmission routes, and safety measures are allowed to work in the lab. This also includes external personnel working temporarily.
  • Use gloves and safety goggles when risk for splashing.
  • Wash your hands with ethanol.
  • Disinfectants are used to disinfect surfaces and equipment. If blood is spilled, clean immediately!
  • All blood-containing liquids are to be poured in a beaker containing 1/10 chlorine (add chlorine last). Leave for at least 15 min, then pour the liquid in the sink in the fume hood and rinse thoroughly. If needed, clean the sink, and clean with disinfectant. When you leave the fume hood it should be clean and tidy.
  • Wash the rotor racks/chambers every day if samples are extracted manually, either manually or in the dishwashing machine. Use disinfectant to clean the inside of the centrifuge.
  • Measures to be taken if injured and while risk of contamination: see below.

4.5 Measures when splashing dangerous compounds into eye

Always use eye protection (protective goggles or shield) when working with dangerous substances. If splashing in the eye occurs anyway, please take the following measures:

Person affectedColleagues
Find an eye shower as quick as possible.Help your colleague find an eye shower and start it.
Call for help. 
Rinse your eye/eyes throughly with cold water for 5-10 min.Help your colleague to rinse the eyes and to keep eyes open while rinsing.
 Arrange with transport to the hospital, 
 Call and inform the eye clinic.
 If needed use drops of sterile sodium chloride solution during transport.

4.6 Measures to be taken if cut injury

Rinse immediately and thoroughly with water and soap, followed by 70% disinfectant. If you suspect exposure to contaminated blood, immediately inform your nearest supervisor and safety inspector.

All cut and stick wounds should be reported to the employer, i.e. nearest supervisor, as an incident without serious injury on the homepage of Arbetsmiljöverket. From there, an automatic notification will be sent to Försäkringskassan.

The report of the injury should be made by the employer, together with the safety inspector and the affected person, and all should get a copy of the report. The report should be made in order to prove an eventual later infection, and give a basis for economical compensation. A zero sample should be taken by the Företagshälsovården immediately.

Also see the instructions from Previa regarding the routines when having an incident with blood contamination risk:

http://interwebben.ki.se/sv/previa-foretagshalsovard

4.7 Telephone list

  • Guard at KI: 070-371 44 00
  • Eye clinic, S:t Eriks: 08-672 30 00; 08-672 31 00 (After 16:00)
  • Fire brigade and ambulance: 112
  • Poison information: 08-33 12 31
  • If a large involuntary emission/contamination has been made to the drain or air, the Miljöenheten (Environmental Unit), municipality, and possibly the avloppsverk should be informed:
    • Miljöenheten: 08-524 866 22
    • KI alarm: 08- 524 80 100
    • Solna kommun (municipality): 08-734 20 00
    • Käppalaverket: 08-766 67 00

References

Kvalitetsmanualen (the quality guidelines)

http://internwebben.ki.se/sites/default/files/blood_rules_ki_rev_enl_afs_2012_7_0.pdf

Internal documents on the homepage of KI:

Collected documents on working environment, fire safety, environmental issues, safety issues, and radiation safety: http://internwebben.ki.se/sv/arbetsmiljo-och-halsa

AFS 1997:7 “Gaser” (gases)

AFS 2004:4 “Kemiska arbetsmiljörisker” (risks working with chemicals)

AFS 1986:23 “Skydd mot blodsmitta” (protection against blood contaminants)

 

 

 

 

 

 

 

 

 

 

 

Citation

SOP collection, KI Biobank. 0352 Guidelines working in the lab - KI Biobank. The Molecular Methods Database. 2016/01/25

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