0314 DNA-freezer

Summary

A description of the maintenance of DNA freezers At KI Biobank. A good maintenance will keep the temperature of the freezer steady and the freezer will work better.

Purpose

Describing the maintenance of the DNA freezers of the Biobank. A good maintenance will keep the temperature of the freezer steady and the freezer will work better.

Scope

The DNA freezers of the Biobank intended for normalized DNA.

Responsibility

  • The staff at KI Biobank is responsible for following this instruction.
  • The Biomedical analyst issuing the instruction is responsible for updating it.

Reference document

Manual: Kirsch Model Froster – 720 Vers. Nr. 184

Instruction: 0293 Larm och Beredskap (alarm and preparedness)

Maintenance and service

Documentation

All the weekly maintenance should be documented in the log sheet for DNA freezers. Service, maintenance every third month, and yearly maintenance should be documented in the Event-log.

Weekly maintenance

Every week the temperature of the freezer should be checked and noted in the log sheet for DNA freezers. For the Kirsch freezers of the Biobank, placed on level 3, the maintenance is done when needed, but at least every second week.

 

Name:

Interval:

Freezer -25°C

-23°C – -27°C

 

When the temperature is outside the given interval, it shall be adjusted, see the manufacturer’s manual. Document any action taken in the Event-log for DNA freezers.

Every sixth month

Testing the alarm of the freezer

Every sixth month the alarm of the freezer shall be tested, see instruction 0293 Larm och Beredskap (alarm and preparedness).

Yearly maintenance

  • Cleaning the compressor, see manual.
  • The condenser is on top of the freezer. Carefully vacuum-clean it.
  • The freezer is self-defrosting.

Moving the freezer

When a freezer is moved this should be noted in the Event-log and the temperature should be logged afterwards. Also, the alarm id of the freezer should be documented in the Event-log.

 

Citation

SOP collection, KI Biobank. 0314 DNA-freezer. The Molecular Methods Database. 2016/01/20

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Procedures and protocols from Karolinska instutet Biobank
Topics covered by Biobank Standard Operating Procedures: